Международная научная периодика
УДК 579.6
РОСТОСТИМУЛИРУЮЩАЯ АКТИВНОСТЬ РИЗОБАКТЕРИЙ BACILLUS CEREUS 25К И BACILLUS SUBTILIS 35A В УСЛОВИЯХ СОЛЕВОГО СТРЕССА
Страницы: 13-19
Кадырова Гульчехра Хакимовна
доктор биологических наук, ведущий научный сотрудник
Института микробиологии Академии наук Республики Узбекистан,
SPIN-код: 7950-4294
Усмонкулова Азиза Анвар кизи
PhD, старший научный сотрудник Научно-исследовательского
института защиты и карантина растений Республики Узбекистан
Шонахунов Тулкин Эркинович
PhD, младший научный сотрудник Института микробиологии Академии наук
Республики Узбекистан
Аннотация:
В статье представлены результаты исследования, целью которого было изучение влияния ростостимулирующих ризобактерий Bacillus cereus 25К и Bacillus subtilis 35A на рост и развитие пшеницы (Triticum aestivum L.) сорта «Чиллаки», а также продукцию ИУК, гиббереллина, ЭПС в условиях солевого стресса. В работе показано, что инокуляция семян пшеницы (Triticum aestivum L.) сорта «Чиллаки» местными штаммами ризобактерий B. cereus 25К и B. subtilis 35A стимулирует всхожесть и рост – развитие проростков в условиях солевого стресса (100 мМ, NaCl). Следовательно, при обработке семян растений ризобактериями B. subtilis 35A увеличивает длину корня и стебля в 1,3 и 1,7 раза в условиях солевого стресса, по сравнению с контролем. На 7 сутки культивирования в условиях солевого стресса (200 мМ, NaCl) продуцирование индолил-3-уксусной кислоты (ИУК) у штаммов B. cereus 25К и B. subtilis 35A увеличивается по сравнению к контрольному варианту в 1,26 и 1,3 раза, соответственно. Также было выявлено, что ростостимулирующие штаммы B. cereus 25К и B. subtilis 35A в течение 7 сут культивирования в условиях солевого стресса (200 мМ, NaCl) продуцируют на 39,2% и 45,4% больше ЭПС по сравнению с контролем.
Ключевые слова:
ризобактерии, пшеница, Bacillus cereus, Bacillus subtilis, инокуляция, солевой стресс, всхожесть, индолил-3уксусная кислота, гиббереллины, экзополисахариды.
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Сведения о финансировании:
Исследование выполнено за счет основного-базового финансирования
Академии Наук Республики Узбекистан.
Ссылка для цитирования:
Кадырова, Г.Х., Усмонкулова, А.А., Шонахунов, Т.Э. Ростостимулирующая активность ризобактерий Bacillus Cereus 25к и Bacillus Subtilis 35a в условиях солевого стресса// Международный научный сельскохозяйственный журнал. – 2025. — №2. – С. 13-19. — https://naukalib.ru/1322/.
GROWTH-PROMOTING ACTIVITY OF RHIZOBACTERIA BACILLUS CEREUS 25K AND BACILLUS SUBTILIS 35A UNDER SALT STRESS
Kadirova Gulchekhra Khakimovna
Doctor of Biological Sciences, Leading Researcher, Institute of Microbiology,
Academy of Sciences of the Republic of Uzbekistan,
SPIN code: 7950-4294
Usmonkulova Aziza Anvar kizi,
PhD, Senior Researcher, Research Institute of Plant Protection and Quarantine,
Uzbekistan
Shonakhunov Tulkin Erkinovich,
PhD, Junior Researcher, Institute of Microbiology, Academy of Sciences
of the Republic of Uzbekistan
Abstract:
The article presents the results of the study, the aim of which was to investigate the effect of growth-promoting rhizobacteria Bacillus cereus 25K and Bacillus subtilis 35A on the growth and development of wheat (Triticum aestivum L.) variety «Chillaki», as well as the production of IAA, gibberellin, exopolysaccharides (EPS) under salt stress. The work shows that inoculation of wheat (Triticum aestivum L.) seeds of the «Chillaki» variety with local strains of rhizobacteria Bacillus cereus 25K and Bacillus subtilis 35A stimulates germination and growth — development of seedlings under salt stress (100 mM, NaCl). Therefore, when treating plant seeds with rhizobacteria B. subtilis 35A increases the length of the root and stem by 1.3 and 1.7 times under salt stress, compared with the control. On the 7th day of cultivation under salt stress conditions (200 mM NaCl), the production of indole-3-acetic acid (IAA) in the B. cereus 25K and B. subtilis 35A strains increased by 1.26 and 1.3 times, respectively, compared to the control variant. It was also found that the growth-promoting strains B.cereus 25K and B. subtilis 35A produced 39.2% and 45.4% more EPS during 7 days of cultivation under salt stress conditions (200 mM NaCl) compared to the control.
Keywords:
rhizobacteria, wheat, Bacillus cereus, Bacillus subtilis, inoculation, salt stress, germination, indole-3-acetic acid, gibberellins, exopolysaccharides.
